Below are PDF file links for each Required Practical Method Sheet.

1. Investigation into the effect of a named variable on the rate of an enzyme-controlled reaction

2. Preparation of stained squashes of cells from plant root tips

3. Production of a dilution series of a solute to produce a calibration curve to identify the water potential of plant tissue

4. Investigation into the effect of a named variable on the permeability of cell-surface membranes

5. Dissection of animal or plant gas exchange or mass transport system or of organ within such a system

6. Use of aseptic techniques to investigate the effect of antimicrobial substances on microbial growth

7. Using chromatography to investigate leaf pigments

8. Factors affecting dehydrogenase activity in chloroplasts

9. Factors affecting the rate of respiration in single-celled organisms

10. The effect of an environmental variable on animal movement

11. Using a calibration curve to identify an unknown concentration